Paperback : $283.00
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls.
Part I: Introduction to Yeast Surface Display and Its Applications
1. Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Maryam Raeeszadeh-Sarmazdeh and Eric T. Boder
Part II: Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants
2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Byong H. Kang, Brianna M. Lax, and K. Dane Wittrup
3. Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations
Benedikt Dolgikh and Daniel Woldring
4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution
James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, and Daniel Woldring
5. Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries
Mehrsa Mardikoraem and Daniel Woldring
6. Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders
Nicole J. Yang
7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display
Preeti Sharma, Erik Procko, and David M. Kranz
8. Engineering Binders with Exceptional Selectivity
Kai Wen Teng, Akiko Koide, and Shohei Koide
9. Affinity and Stability Analysis of Yeast Displayed Proteins
Charlotte U. Zajc, Magdalena Teufl, and Michael W. Traxlmayr
Part III: Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix
10. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, and Harald Kolmar
11. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins
Patrick J. Krohl and Jamie B. Spangler
12. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques
Rojhae A. Panton and Lawrence A. Stern
13. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Benjamin J. Umlauf, John S. Kuo, and Eric V. Shusta
Part IV: Specialized Yeast Surface Display Applications
14. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology
15. Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors
Brooke D. Huisman, Beth E. Grace, Patrick V. Holec, and Michael E. Birnbaum
16. Yeast Display Guided Selection of pH Dependent Binders
Jenna N. Meanor, Albert J. Keung, Balaji M. Rao, and Nimish Gera
17. Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies
Du-San Baek, Seong-Wook Park, Cynthia Adams, Dimiter S. Dimitrov, and Yong-Sung Kim
18. Humanization of Chicken-Derived Antibodies by Yeast Surface Display
Jan P. Bogen, Adrian Elter, Julius Grzeschik, Björn Hock, and Harald Kolmar
19. Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display
Mari R. Toumaian and Maryam Raeeszadeh-Sarmazdeh
20. Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries
Kaitlyn Bacon, Stefano Menegatti, and Balaji M. Rao
21. Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel Tay, Say Yong Ng, and Hadley D. Sikes
22. Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries
Alec A. Desai, Jennifer M. Zupancic, Matthew D. Smith, and Peter M. Tessier
23. Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface
Rebecca Hershman, Arlinda Rezhdo, Jessica Stieglitz, and James A. Van Deventer
24. Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles
Stefan Vogt, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Stracke, Madhusudhan Reddy Bobbili, Johannes Grillari, Florian Rüker, and Gordana Wozniak-Knopp
25. Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display
Lina El Hajji, Hela Benaissa, and Arnaud Gautier
26. Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls.
Part I: Introduction to Yeast Surface Display and Its Applications
1. Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Maryam Raeeszadeh-Sarmazdeh and Eric T. Boder
Part II: Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants
2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Byong H. Kang, Brianna M. Lax, and K. Dane Wittrup
3. Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations
Benedikt Dolgikh and Daniel Woldring
4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution
James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, and Daniel Woldring
5. Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries
Mehrsa Mardikoraem and Daniel Woldring
6. Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders
Nicole J. Yang
7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display
Preeti Sharma, Erik Procko, and David M. Kranz
8. Engineering Binders with Exceptional Selectivity
Kai Wen Teng, Akiko Koide, and Shohei Koide
9. Affinity and Stability Analysis of Yeast Displayed Proteins
Charlotte U. Zajc, Magdalena Teufl, and Michael W. Traxlmayr
Part III: Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix
10. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, and Harald Kolmar
11. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins
Patrick J. Krohl and Jamie B. Spangler
12. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques
Rojhae A. Panton and Lawrence A. Stern
13. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Benjamin J. Umlauf, John S. Kuo, and Eric V. Shusta
Part IV: Specialized Yeast Surface Display Applications
14. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology
15. Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors
Brooke D. Huisman, Beth E. Grace, Patrick V. Holec, and Michael E. Birnbaum
16. Yeast Display Guided Selection of pH Dependent Binders
Jenna N. Meanor, Albert J. Keung, Balaji M. Rao, and Nimish Gera
17. Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies
Du-San Baek, Seong-Wook Park, Cynthia Adams, Dimiter S. Dimitrov, and Yong-Sung Kim
18. Humanization of Chicken-Derived Antibodies by Yeast Surface Display
Jan P. Bogen, Adrian Elter, Julius Grzeschik, Björn Hock, and Harald Kolmar
19. Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display
Mari R. Toumaian and Maryam Raeeszadeh-Sarmazdeh
20. Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries
Kaitlyn Bacon, Stefano Menegatti, and Balaji M. Rao
21. Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel Tay, Say Yong Ng, and Hadley D. Sikes
22. Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries
Alec A. Desai, Jennifer M. Zupancic, Matthew D. Smith, and Peter M. Tessier
23. Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface
Rebecca Hershman, Arlinda Rezhdo, Jessica Stieglitz, and James A. Van Deventer
24. Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles
Stefan Vogt, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Stracke, Madhusudhan Reddy Bobbili, Johannes Grillari, Florian Rüker, and Gordana Wozniak-Knopp
25. Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display
Lina El Hajji, Hela Benaissa, and Arnaud Gautier
26. Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions
Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System.- Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.- Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations.- Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution.- Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries.- Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders.- Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display.- Engineering Binders with Exceptional Selectivity.- Affinity and Stability Analysis of Yeast Displayed Proteins.- Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting.- A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins.- Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques.- Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.- Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology.- Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors.- Yeast Display Guided Selection of pH Dependent Binders.- Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies.- Humanization of Chicken-Derived Antibodies by Yeast Surface Display.- Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display.- Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries.- Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.- Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries.- EngineeringProteins Containing Noncanonical Amino Acids on the Yeast Surface.- Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles.- Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display.- Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions.
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