Table of Contents

1. High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy
Renpeng Ding, Cheng-Chi Chao and Qianqian Gao
2. Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice
Hairuo Wen, Ying Huang, Tiantian Hou, Junzhi Wang and Yan Huo
3. Generation of CAR-T cells using lentiviral vectors
Mansour Poorebrahim, Isaac Quiros-Fernandez, Elham Fakhr and Angel Cid-Arregui
4. Phenotyping of CAR T cells
Sanjay Rathod
5. Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis
Amanda N. Lisby, Robert D. Carlson, Trevor R. Baybutt, Megan Weindorfer and Adam E. Snook
6. Flow cytometry detection and quantification of CAR T cells into solid tumors
Nicholas J.A. Tokarew, Javier Suárez Gosálvez, Alessia Nottebrock, Daria Briukhovestka, Stefan Endres, Bruno L. Cadilha and Sebastian Kobold
7. Generation and functional characterization of CAR exosomes
Chuqi Wang, Wenyan Fu, Changhai Lei and Shi Hu
8. Preclinical testing of CAR T cells in zebrafish xenografts
Sarah Grissenberger, Benjamin Salzer, Susana Pascoal, Andrea Wenninger-Weinzierl, Manfred Lehner and Martin Distel
9. In vivo experimental mouse model to test CD19 CAR T cells generated with different methods
Anne Fåne, Marit Renée Myhre, Else Marit Inderberg and Sébastien Wälchli
10. Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy
Marcelo de Souza Fernandes Pereira, Aarohi Thakkar and Dean Anthony Lee
11. Generation of CAR T-cells using γ-retroviral vector
Norihiro Watanabe and Mary Kathryn McKenna
12. Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models
Mohammad Alhomoud, Jeremie Martinet, Mayumi Sugita, Alexandra Gomez-Arteaga and Monica L. Guzman
13. Strategies for manufacturing cell therapy products aligned with patient needs
Prajna Guha and Steven C. Katz

About the Author

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Sheila Spada is at Weill Cornell Medical College, NY, USA