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Eosinophils
Methods and Protocols (Methods in Molecular Biology)
By Garry M. Walsh (Edited by)

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Format
Paperback, 324 pages
Other Formats Available

Hardback : $166.00

Published
United States, 1 August 2016

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.¿ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.



1.                  Eosinophil Overview  -  Structure, Biological Properties And Key Functions

Paige Lacy, Helene F. Rosenberg, Garry M. Walsh


2.                  Eosinophil Purification From Peripheral Blood

Praveen Akuthota, Kelsey Capron And Peter F. Weller


3.                  Eosinophil Purification From Human Bone Marrow

Tina W. Wong And Diane F. Jelinek


4.                  CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung

Apostolos Bossios And Madeleine Rådinger


5.                  Eosinophil Cell Lines

Kenji Ishihara


6.                  Cell Signalling In Eosinophil During Human Eosinophil Differentiation

Miranda Buitenhuis


7.                  Eosinophil Intracellular Signalling - Apoptosis

 Pinja Ilmarinen, Eeva Moilanen And Hannu Kankaanranta


8.                  Identification Of Human Eosinophils In Whole Blood By Flow Cytometry

Caroline Ethier, Paige Lacy And Francis Davoine


9.                  Isolation And Functional Assessment Of Eosinophil Crystalloid Granules

Renata Baptista-Dos-Reis, Valdirene S. Muniz And Josiane S. Neves


10.              Eosinophil Chemotaxis

Gordon Dent


11.              Human Eosinophil Shape Change And Secretion

Lian Willetts And Paige Lacy


12.              Human Eosinophil Adhesion And Receptor Expression

Colleen S. Curran


13.              Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions

Viktoria Konya,  Miriam Peinhaupt And Akos Heinemann


14.              Human Eosinophil Transmigration

Stanislawa Bazan-Socha, Joanna Zuk, Bogdan Jakiela And Jacek Musial


15.              Measurement Of Eosinophil Kinetics In Healthy Volunteers

Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Daniel Gillett, Sarah Heard, A. Michael Peters, Alison M. Condliffe And Edwin R. Chilvers


16.              Assays Of Eosinophil Apoptosis And Phagocytic Uptake

David A. Dorward, Sidharth Sharma, Ana L. Alessandri, Adriano G. Rossi, Christopher D. Lucas


17.              Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis

Osmo Kari And K. Matti Saari


18.              Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections

Masataka Korenaga And Fabrizio Bruschi


19.              Interactions Of Eosinophils With Nerves

Quinn R. Roth-Carter, David B. Jacoby And Zhenying Nie


20.              Eosinophils Interaction With Mast Cells: The Allergic Effector Unit

Roopesh Singh Gangwar And Francesca Levi-Schaffer


21.              Eosinophil Interactions: Antigen Presentation

Praveen Akuthota


22.              Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue

Caroline M. Percopo, Kimberly D. Dyer, Kendal A. Karpe, Joseph B. Domachowske And Helene F. Rosenberg


23.              Antimicrobial Activity Of Human Eosinophil Granule Proteins

Anu Chopra, And Janendra K. Batra


24.              Eosinophils And The Ovalbumin Mouse Model Of Asthma

F. Daubeuf And N. Frossard


25.              Mutant Mice And Animal Models Of Allergic Disease

Marie-Renée Blanchet, Matthew Gold And Kelly M Mcnagny


26.              Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa  Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis

Yoshiyuki Yamada, Jose A. Cancelas And Marc E. Rothenberg

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Product Description

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.¿ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.



1.                  Eosinophil Overview  -  Structure, Biological Properties And Key Functions

Paige Lacy, Helene F. Rosenberg, Garry M. Walsh


2.                  Eosinophil Purification From Peripheral Blood

Praveen Akuthota, Kelsey Capron And Peter F. Weller


3.                  Eosinophil Purification From Human Bone Marrow

Tina W. Wong And Diane F. Jelinek


4.                  CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung

Apostolos Bossios And Madeleine Rådinger


5.                  Eosinophil Cell Lines

Kenji Ishihara


6.                  Cell Signalling In Eosinophil During Human Eosinophil Differentiation

Miranda Buitenhuis


7.                  Eosinophil Intracellular Signalling - Apoptosis

 Pinja Ilmarinen, Eeva Moilanen And Hannu Kankaanranta


8.                  Identification Of Human Eosinophils In Whole Blood By Flow Cytometry

Caroline Ethier, Paige Lacy And Francis Davoine


9.                  Isolation And Functional Assessment Of Eosinophil Crystalloid Granules

Renata Baptista-Dos-Reis, Valdirene S. Muniz And Josiane S. Neves


10.              Eosinophil Chemotaxis

Gordon Dent


11.              Human Eosinophil Shape Change And Secretion

Lian Willetts And Paige Lacy


12.              Human Eosinophil Adhesion And Receptor Expression

Colleen S. Curran


13.              Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions

Viktoria Konya,  Miriam Peinhaupt And Akos Heinemann


14.              Human Eosinophil Transmigration

Stanislawa Bazan-Socha, Joanna Zuk, Bogdan Jakiela And Jacek Musial


15.              Measurement Of Eosinophil Kinetics In Healthy Volunteers

Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Daniel Gillett, Sarah Heard, A. Michael Peters, Alison M. Condliffe And Edwin R. Chilvers


16.              Assays Of Eosinophil Apoptosis And Phagocytic Uptake

David A. Dorward, Sidharth Sharma, Ana L. Alessandri, Adriano G. Rossi, Christopher D. Lucas


17.              Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis

Osmo Kari And K. Matti Saari


18.              Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections

Masataka Korenaga And Fabrizio Bruschi


19.              Interactions Of Eosinophils With Nerves

Quinn R. Roth-Carter, David B. Jacoby And Zhenying Nie


20.              Eosinophils Interaction With Mast Cells: The Allergic Effector Unit

Roopesh Singh Gangwar And Francesca Levi-Schaffer


21.              Eosinophil Interactions: Antigen Presentation

Praveen Akuthota


22.              Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue

Caroline M. Percopo, Kimberly D. Dyer, Kendal A. Karpe, Joseph B. Domachowske And Helene F. Rosenberg


23.              Antimicrobial Activity Of Human Eosinophil Granule Proteins

Anu Chopra, And Janendra K. Batra


24.              Eosinophils And The Ovalbumin Mouse Model Of Asthma

F. Daubeuf And N. Frossard


25.              Mutant Mice And Animal Models Of Allergic Disease

Marie-Renée Blanchet, Matthew Gold And Kelly M Mcnagny


26.              Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa  Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis

Yoshiyuki Yamada, Jose A. Cancelas And Marc E. Rothenberg

Show more
Product Details
EAN
9781493955091
ISBN
1493955098
Publisher
Other Information
Illustrated
Dimensions
25.4 x 17.8 x 1.8 centimeters (6.32 kg)

Table of Contents

Eosinophil Overview - Structure, Biological Properties And Key Functions.- Eosinophil Purification From Peripheral Blood.- Eosinophil Purification From Human Bone Marrow.- CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung.- Eosinophil Cell Lines.- Cell Signalling In Eosinophil During Human Eosinophil Differentiation.- Eosinophil Intracellular Signalling – Apoptosis.- Identification Of Human Eosinophils In Whole Blood By Flow Cytometry.- Isolation And Functional Assessment Of Eosinophil Crystalloid Granules.- Eosinophil Chemotaxis.- Human Eosinophil Shape Change And Secretion.- Human Eosinophil Adhesion And Receptor Expression.- Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions.- Human Eosinophil Transmigration.- Measurement Of Eosinophil Kinetics In Healthy Volunteers.- Assays Of Eosinophil Apoptosis And Phagocytic Uptake.- Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis.- Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections.- Interactions Of Eosinophils With Nerves.- Eosinophils Interaction With Mast Cells: The Allergic Effector Unit.- Eosinophil Interactions: Antigen Presentation.- Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue.- Antimicrobial Activity Of Human Eosinophil Granule Proteins.- Eosinophils And The Ovalbumin Mouse Model Of Asthma.- Mutant Mice And Animal Models Of Allergic Disease.- Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis.

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