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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium

Table of Contents

Electron microscopy of IFT in cilia and flagella

Biochemical analysis of ciliary transport
Visualization of IFT in Chlamydomonas flagella
Visualization of kidney cilia-mediated calcium signaling
Methods for analyzing nodal cilia and flow
Methods for building synthetic cilia-like motor-microtubule arrays
Isolation of IFT particle proteins from Chlamydomonas
Radial Spoke isolation and assays
Biomechanical measurements of kinocilium
Methods for analysis of Calcium/calmodulin signaling in flagella
Ubiquitination of Flagellar Proteins
Visualizing IFT in C. elegans cilia
Proteomic analysis of mammalian primary cilia
TSC and TOR signaling in cilia
Bioinformatic approaches to cilia
Methods for testing cilia function in cell migration
Signaling and waveform in Drosophila sperm flagella
Analysis of import into primary cilia
Patch-Clamp Recording from Mouse and Human Spermatozoa
Optical trap measurements of flagellar membrane motility

About the Author

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.